blue-white colour selection
- blue-white colour selection
-
Method for identifying bacterial clones containing plasmids with inserts. Many modern vectors have their
polycloning site within
a part of the
LacZ gene encoding
beta-galactosidase, which provides
a -
complementation in an appropriate mutant
E. coli strain. This means that
a re-ligated (empty)
vector will produce blue colonies when grown on plates containing
IPTG and
Xgal, but colonies with
a substantial insert in their plasmid\'s
polycloning site are unable to produce functional beta-galactosidase, and so produce
white colonies.
Dictionary of molecular biology.
2004.
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